Cold-water coral reef frameworks, megafaunal communities and evidence for coral carbonate mounds on the Hatton Bank, north east Atlantic

Offshore banks and seamounts sustain diverse megafaunal communities, including framework reefs formed by cold-water corals. Few studies have quantified environmental effects on the alpha or beta diversity of these communities. We adopted an interdisciplinary approach that used historical geophysical data to identify topographic highs on Hatton Bank, which were surveyed visually. The resulting photographic data were used to examine relationships between megafaunal communities and macrohabitat, the latter defined into six categories (mud, sand, cobbles, coral rubble, coral framework, rock). The survey stations revealed considerable small-scale variability in macrohabitat from exposed Late Palaeocene lava flows to quiescent muddy habitats and coral-built carbonate mounds. The first reported evidence for coral carbonate mound development in UK waters is presented, which was most pronounced near present-day or former sites of topographic change, suggesting that local current acceleration favoured coral framework growth and mound initiation. Alpha diversity varied significantly across macrohabitats, but not between rock and coral rubble, or between smaller grain sized categories of cobbles, sand and mud. Community composition differed between most macrohabitats, and variation in beta diversity across Hatton Bank was largely explained by fine-scale substratum. Certain megafauna were clearly associated with particular macrohabitats, with stylasterid corals notably associated with cobble and rock habitats and coral habitats characterized by a diverse community of suspension-feeders. The visual surveys also produced novel images of deep-water megafauna including a new photographic record of the gorgonian coral Paragorgia arborea, a species not previously reported from Rockall Plateau. Further interdisciplinary studies are needed to interpret beta diversity across these and other environmental gradients on Hatton Bank. It is clear that efforts are also needed to improve our understanding of the genetic connectivity and biogeography of vulnerable deep-water ecosystems and to develop predictive models of their occurrence that can help inform future conservation measures

Expanding Dimensionality in Cinema Color: Impacting Observer Metamerism through Multiprimary Display

Television and cinema display are both trending towards greater ranges and saturation of reproduced colors made possible by near-monochromatic RGB illumination technologies. Through current broadcast and digital cinema standards work, system designs employing laser light sources, narrow-band LED, quantum dots and others are being actively endorsed in promotion of Wide Color Gamut (WCG). Despite artistic benefits brought to creative content producers, spectrally selective excitations of naturally different human color response functions exacerbate variability of observer experience. An exaggerated variation in color-sensing is explicitly counter to the exhaustive controls and calibrations employed in modern motion picture pipelines. Further, singular standard observer summaries of human color vision such as found in the CIE’s 1931 and 1964 color matching functions and used extensively in motion picture color management are deficient in recognizing expected human vision variability. Many researchers have confirmed the magnitude of observer metamerism in color matching in both uniform colors and imagery but few have shown explicit color management with an aim of minimized difference in observer perception variability. This research shows that not only can observer metamerism influences be quantitatively predicted and confirmed psychophysically but that intentionally engineered multiprimary displays employing more than three primaries can offer increased color gamut with drastically improved consistency of experience. To this end, a seven-channel prototype display has been constructed based on observer metamerism models and color difference indices derived from the latest color vision demographic research. This display has been further proven in forced-choice paired comparison tests to deliver superior color matching to reference stimuli versus both contemporary standard RGB cinema projection and recently ratified standard laser projection across a large population of color-normal observers

Development of a SMA-Based, Slat-Gap Filler for Airframe Noise Reduction

Noise produced by unsteady flow around aircraft structures, termed airframe noise, is an important source of aircraft noise during the approach and landing phases of flight. Conventional leading-edge-slat devices for high lift on typical transport aircraft are a prominent source of airframe noise. Many concepts for slat noise reduction have been investigated. Slat-cove fillers have emerged as an attractive solution, but they maintain the gap flow, leaving some noise production mechanisms unabated, and thus represent a nonoptimal solution. Drooped-leading-edge (DLE) concepts have been proposed as "optimal" because the gap flow is eliminated. The deployed leading edge device is not distinct and separate from the main wing in DLE concepts and the high-lift performance suffers at high angles of attack (alpha) as a consequence. Elusive high-alpha performance and excessive weight penalty have stymied DLE development. The fact that high-lift performance of DLE systems is only affected at high alpha suggests another concept that simultaneously achieves the high-lift of the baseline airfoil and the noise reduction of DLE concepts. The concept involves utilizing a conventional leading-edge slat device and a deformable structure that is deployed from the leading edge of the main wing and closes the gap between the slat and main wing, termed a slat-gap filler (SGF). The deployable structure consists of a portion of the skin of the main wing and it is driven in conjunction with the slat during deployment and retraction. Benchtop models have been developed to assess the feasibility and to study important parameters. Computational models have assisted in the bench-top model design and provided valuable insight in the parameter space as well as the feasibility

Modeling the Random Component of Manufacturing Yield of Integrated Circuits.

A model is created for the number of integrated circuits that are good from each wafer on which they are fabricated. The goal is to separate the random or common cause loss from the systematic or special loss. The random loss from this type of process is modeled so that false alarms indicating systematic loss are less likely to occur and so that the structure of the systematic loss can be determined

Analysis of noncanonical calciumdependent protein kinases in Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9

Calcium-dependent protein kinases (CDPKs) are expanded in apicomplexan parasites, especially in Toxoplasma gondii where 14 separate genes encoding these enzymes are found. Although previous studies have shown that several CDPKs play a role in controlling invasion, egress, and cell division in T. gondii, the roles of most of these genes are unexplored. Here we developed a more efficient method for gene disruption using CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) that was modified to completely delete large, multiexonic genes from the genome and to allow serial replacement by recycling of the selectable marker using Cre-loxP. Using this system, we generated a total of 24 mutants in type 1 and 2 genetic backgrounds to ascertain the functions of noncanonical CDPKs. Remarkably, although we were able to confirm the essentiality of CDPK1 and CDPK7, the majority of CDPKs had no discernible phenotype for growth in vitro or infection in the mouse model. The exception to this was CDPK6, loss of which leads to reduced plaquing, fitness defect in a competition assay, and reduced tissue cyst formation in chronically infected mice. Our findings highlight the utility of CRISPR/Cas9 for rapid serial gene deletion and also suggest that additional models are needed to reveal the functions of many genes in T. gondii